The Mayne Academy is headed by Professor Darrell Crawford and encompasses teaching and research in all aspects of medicine. This includes the various internal body systems, such as haematology, cardiology, endocrinology, geriatric medicine, gastroenterology, renal medicine, clinical pharmacology, respiratory medicine, general medicine, rheumatology, neurology, and immunology. The Mayne Academy's academic staff members are involved in education, patient care, research, and community service.

All of the Mayne Academy's teaching staff are medical specialists and have concurrent Queensland Health appointments in hospitals, many as Directors of their specialty in the hospitals in which they work.